M-MLV Reverse Transcriptase RNase H-


For in vitro use only


Description:

The M-MLV Reverse Transcriptase RNase H- is a genetically modified M-MLV Reverse Transcriptase which exhibits RNA or DNA dependent DNA polymerase, but lacks ribonuclease H activity. This enzyme can synthesize a complementary DNA strand initiating from a primer using RNA or DNA templates. Removal of RNase H activity results in an increase of full-length cDNA products



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Products
Info
Data Sheet
Cat. No. Pack Size Conc.
06-21-050000 50000 U 200 U/µl
06-21-200000 200000 U 200 U/µl
06-21-010000 10000 U 200 U/µl
Applications:

- RNA analysis by primer extension

- DNA labeling

- cDNA synthesis

Concentration:

200 U/µl

Reagents Provided:

- M-MLV Reverse Transcriptase RNase-

- 5x Reaction Buffer 1 (With MgCl2 and DTT)
0.25 M Tris-HCl, 0.5 M KCl, 30mM MgCl2, 25 mM DTT

- 5x Reaction Buffer 2 (MgCl2 and DTT free)
0.25 M Tris-HCl, 0.5 M KCl

- 25 mM MgCl2

- 20 mM MnCl

-100 mM DDT

Storage and Dilution Buffer:

50% glycerol (v/v), 50 mM Tris-HCl pH 8.0 at 25°C, 100 mM NaCl, 0.1 mM EDTA, 0.1% NP-40

Shipping and Storage Conditions:

Shipping and storage at room temperature has no detrimental effects on the quality M-MLV Reverse Transcriptase RNase H-. However you may store at -20°C for your own convinence.

For in vitro use only