5x HOT FIREPol® Probe qPCR Mix Plus (no ROX)

For in vitro use only


5x HOT FIREPol® Probe qPCR Mix Plus (no ROX) is optimized for real-time quantitative PCR assays using sequence-specific fluorogenic DNA-probe chemistries. The Mix contains all the components necessary to perform qPCR, with the exception of template, primers, and probe. The qPCR Mix contains optimized components and HOT FIREPol® DNA Polymerase supplied in a proprietary reaction buffer that enables detection of low copy number targets. HOT FIREPol® DNA Polymerase is activated by a 15 min incubation step at 95°C. This prevents extension of non-specifically annealed primers and primer-dimers formed at low temperatures during qPCR setup.

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Data Sheet
Cat. No. Pack Size Conc.
08-15-00008 8 ml 5x
08-15-00001 1 ml 5x
08-15-00020 20 ml 5x

- Detection and quantification of DNA and cDNA targets

- Profiling gene expression

- Microbial detection

- Viral load determination

Mix Compatibility:

- Bio-Rad: CFX96TM & CFX384TM, iQTM5 & MyiQTM, Chromo4TM, Opticon® 2 & MiniOpticon®

- Qiagen: Rotor-Gene® Q, Rotor-Gene® 6000

- Eppendorf: Mastercycler®: ep realplex2 & ep realplex4

- Illumina: The EcoTM

- Roche: LightCycler® 480

Mix Composition:

- HOT FIREPol® DNA Polymerase

- 5x Probe qPCR buffer

- 15 mM MgCl2 1x PCR solution – 3 mM

- dNTPs blend containing dUTP/dTTP including dTTP to improve reaction sensitivity and efficiency compared to dUTP

- No ROX dye

Shipping and Storage Conditions:

Shipping and storage at room temperature has no detrimental effects on the quality 5x HOT FIREPol® Probe qPCR Mix Plus (no ROX). However you may store at -20°C for your own convinence.

For in vitro use only