5x HOT FIREPol^® Probe qPCR Mix Plus (no ROX)

For in vitro use only

Description:

5x HOT FIREPol^® Probe qPCR Mix Plus (no ROX) is optimized for real-time quantitative PCR assays using sequence-specific fluorogenic DNA-probe chemistries. The Mix contains all the components necessary to perform qPCR, with the exception of template, primers, and probe. The qPCR Mix contains optimized components and HOT FIREPol^® DNA Polymerase supplied in a proprietary reaction buffer that enables detection of low copy number targets. HOT FIREPol^® DNA Polymerase is activated by a 15 min incubation step at 95°C. This prevents extension of non-specifically annealed primers and primer-dimers formed at low temperatures during qPCR setup.

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Info

Data Sheet / MSDS/ COA

Cat. No.	Pack Size	Conc.
08-15-00008	8 ml	5x
08-15-00001	1 ml	5x
08-15-00020	20 ml	5x

Applications:

- Detection and quantification of DNA and cDNA targets

- Profiling gene expression

- Microbial detection

- Viral load determination

Mix Compatibility:

- Bio-Rad: CFX96^TM & CFX384^TM, iQ^TM5 & MyiQ^TM, Chromo4^TM, Opticon^® 2 & MiniOpticon^®

- Qiagen: Rotor-Gene^® Q, Rotor-Gene^® 6000

- Eppendorf: Mastercycler^®: ep realplex2 & ep realplex4

- Illumina: The Eco^TM

- Roche: LightCycler^® 480

Mix Composition:

- HOT FIREPol^® DNA Polymerase

- 5x Probe qPCR buffer

- 15 mM MgCl₂ 1x PCR solution – 3 mM

- dNTPs blend containing dUTP/dTTP including dTTP to improve reaction sensitivity and efficiency compared to dUTP

- No ROX dye

Shipping and Storage Conditions:

Shipping and storage at room temperature has no detrimental effects on the quality 5x HOT FIREPol^® Probe qPCR Mix Plus (no ROX). However you may store at -20^°C for your own convinence.