For in vitro use only
Description:
5x HOT FIREPol® Probe qPCR Mix Plus (no ROX) is optimized for real-time quantitative PCR assays using sequence-specific fluorogenic DNA-probe chemistries. The Mix contains all the components necessary to perform qPCR, with the exception of template, primers, and probe. The qPCR Mix contains optimized components and HOT FIREPol® DNA Polymerase supplied in a proprietary reaction buffer that enables detection of low copy number targets. HOT FIREPol® DNA Polymerase is activated by a 15 min incubation step at 95°C. This prevents extension of non-specifically annealed primers and primer-dimers formed at low temperatures during qPCR setup.
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Cat. No. | Pack Size | Conc. |
---|---|---|
08-15-00008 | 8 ml | 5x |
08-15-00001 | 1 ml | 5x |
08-15-00020 | 20 ml | 5x |
- Detection and quantification of DNA and cDNA targets
- Profiling gene expression
- Microbial detection
- Viral load determination
- Bio-Rad: CFX96TM & CFX384TM, iQTM5 & MyiQTM, Chromo4TM, Opticon® 2 & MiniOpticon®
- Qiagen: Rotor-Gene® Q, Rotor-Gene® 6000
- Eppendorf: Mastercycler®: ep realplex2 & ep realplex4
- Illumina: The EcoTM
- Roche: LightCycler® 480
- HOT FIREPol® DNA Polymerase
- 5x Probe qPCR buffer
- 15 mM MgCl2 1x PCR solution – 3 mM
- dNTPs blend containing dUTP/dTTP including dTTP to improve reaction sensitivity and efficiency compared to dUTP
- No ROX dye
Shipping and storage at room temperature has no detrimental effects on the quality 5x HOT FIREPol® Probe qPCR Mix Plus (no ROX). However you may store at -20°C for your own convinence.
For in vitro use only
Mastercycler is a trademark of Eppendorf AG. Rotor-Gene is a registered trademark of Corbett Research (Qiagen). CFX96, CFX384, iQ, MyiQ, Chromo4, Opticon 2, MiniOpticon are trademarks of Bio-Rad Laboratories. LightCycler is a registered trademark of Roche Applied Sciences. The Eco is registered trademark of Illumina Inc.
Some applications this product is used in may require a license which is not provided by the purchase of this product. Users should obtain the license if required.
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