5x HOT FIREPol® Probe qPCR Mix Plus (Capillary)


For in vitro use only


Description:

5x HOT FIREPol® Probe qPCR Mix Plus (Capillary) is optimized for real-time quantitative PCR assays using sequence-specific fluorogenic DNA-probe chemistries. The Mix contains all the components necessary to perform qPCR, with the exception of template, primers, and probe. The qPCR Mix contains optimized components and HOT FIREPol® DNA Polymerase supplied in a proprietary reaction buffer that enables detection of low copy number targets. HOT FIREPol® DNA Polymerase is activated by a 15 min incubation step at 95°C. This prevents extension of non-specifically annealed primers and primer-dimers formed at low temperatures during qPCR setup.



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Products
Info
Data Sheet
Cat. No. Pack Size Conc.
08-16-00001 1 ml 5x
08-16-00008 8 ml 5x
08-16-00020 20 ml 5x
Applications:

- Detection and quantification of DNA and cDNA targets

- Profiling gene expression

- Microbial detection

- Viral load determination

Mix Compatibility:

- Roche: LightCycler® 1.x, LightCycler® 2.0

Mix Composition:

- HOT FIREPol® DNA Polymerase

- 5x Probe qPCR buffer

- 15 mM MgCl2 1x PCR solution – 3 mM

- dNTPs blend containing dUTP/dTTP including dTTP to improve reaction sensitivity and efficiency compared to dUTP

- BSA

Shipping and Storage Conditions:

Shipping and storage at room temperature has no detrimental effects on the quality 5x HOT FIREPol® Probe qPCR Mix Plus (Capillary). However you may store at -20°C for your own convinence.

For in vitro use only