5x HOT FIREPol® Probe qPCR Mix Plus (ROX)

For in vitro use only


5x HOT FIREPol® Probe qPCR Mix Plus (ROX) is optimized for real-time quantitative PCR assays using sequence-specific fluorogenic DNA-probe chemistries. The Mix contains all the components necessary to perform qPCR, with the exception of template, primers, and probe. The qPCR Mix contains optimized components and HOT FIREPol® DNA Polymerase supplied in a proprietary reaction buffer that enables detection of low copy number targets. HOT FIREPol® DNA Polymerase is activated by a 15 min incubation step at 95°C. This prevents extension of non-specifically annealed primers and primer-dimers formed at low temperatures during qPCR setup.

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Data Sheet
Cat. No. Pack Size Conc.
08-14-00008 8 ml 5x
08-14-00001 1 ml 5x
08-14-00020 20 ml 5x

- Detection and quantification of DNA and cDNA targets

- Profiling gene expression

- Microbial detection

- Viral load determination

Mix Compatibility:

- Applied BioSystems: QuantStudioTM 12K Flex, ViiATM 7, 7900HT, 7500, 7700, StepOneTM & StepOnePlusTM

- Stratagene: MX3000PTM , MX3005PTM

Mix Composition:

- HOT FIREPol® DNA Polymerase

- 5x Probe qPCR buffer

- 15 mM MgCl2 1x PCR solution – 3 mM

- dNTPs

- ROX dye

ROX Dye:

ROX is an internal passive reference dye used to normalize the fluorescent reporter signal generated in qPCR. It does not have any inhibitory effect on cyclers that do not need normalization.

Shipping and Storage Conditions:

Shipping and storage at room temperature has no detrimental effects on the quality 5x HOT FIREPol® Probe qPCR Mix Plus (ROX). However you may store at -20°C for your own convinence.

For in vitro use only