5x HOT FIREPol® EvaGreen® qPCR Supermix

Suitable for ROX-dependent and ROX-independent qPCR cyclers

For in vitro use only


5x HOT FIREPol® EvaGreen® qPCR Supermix is an optimised ready-to-use solution for real-time quantitative PCR assays, including EvaGreen® dye. It comprises of all the components necessary to perform qPCR excluding the template and primers, to perform highly sensitive qPCR. HOT FIREPol® DNA Polymerase is activated by a 12 min incubation step at 95°C. Hot-start mechanism prevents extension of non-specifically annealed primers and primer-dimers formed at low temperatures during qPCR setup.

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Data Sheet
Cat. No. Pack Size Conc.
08-36-00001 1 ml 5x
08-36-00008 8 ml 5x
08-36-00020 20 ml 5x

- Detection and quantification of DNA and cDNA targets

- Profiling gene expression

- Microbial detection

- Viral load determination


- Highly specific and reproducible real time PCR

- Excellent efficiency in case of low copy number targets

- UNG treatment capability due to dNTP blend of dUTP/dTTP

- Superior performance with long (up to 500 bp) and GC-rich templates

- Blue visualisation dye for easy pipetting

Mix Compatibility:

- Applied BioSystems: QuantStudioTM 12K Flex, ViiATM 7, 7900HT, 7500, 7700, StepOneTM & StepOnePlusTM

- Stratagene: MX3000PTM , MX3005PTM

- Bio-Rad: CFX96TM & CFX384TM, iQTM5 & MyiQTM, Chromo4TM, Opticon® 2 & MiniOpticon®

- Qiagen: Rotor-Gene® Q, Rotor-Gene® 6000

- Eppendorf: Mastercycler®: ep realplex2 & ep realplex4

- Illumina: The EcoTM

- Roche: LightCycler® 480

Mix Composition:

- HOT FIREPol® DNA Polymerase

- 5x EvaGreen® qPCR buffer

- 12.5 mM MgCl2 1x PCR solution – 2.5 mM

- dNTPs blend containing dUTP/dTTP including dTTP to improve reaction sensitivity and efficiency compared to dUTP

- EvaGreen® dye

- Internal reference based on ROX dye

- GC-rich Enhancer

- Blue visualisation dye

EvaGreen® Dye:

EvaGreen® is a DNA-binding dye with many features that make it a superior alternative to SYBR® Green I for qPCR. Apart from having similar spectra, EvaGreen® has three important features that set it apart from SYBR® Green I: EvaGreen® has much less PCR inhibition, is extremely stable dye and has been shown to be nonmutagenic and noncytotoxic. EvaGreen® is compatible with all common real-time PCR cyclers – simply select the standard settings for SYBR® Green or FAM!

ROX Dye:

ROX is an internal passive reference dye used to normalize the fluorescent reporter signal generated in qPCR. It does not have any inhibitory effect on cyclers that do not need normalization.

Shipping and Storage Conditions:

Shipping and storage at room temperature has no detrimental effects on the quality 5x HOT FIREPol® EvaGreen® qPCR Supermix. However you may store at -20°C for your own convinence.

For in vitro use only