5x HOT FIREPol® EvaGreen® qPCR Mix Plus (no ROX)


For in vitro use only


Description:

5x HOT FIREPol® EvaGreen® qPCR Mix Plus (no ROX) is an optimised ready-to-use solution for real-time quantitative PCR assays, incorporating EvaGreen® dye. It comprises all the components necessary to perform qPCR: HOT FIREPol® DNA Polymerase, ultrapure dNTPs, MgCl2 and EvaGreen® dye. The user simply needs to add water, template and primers. HOT FIREPol®DNA Polymerase is activated by a 15 min incubation step at 95°C. This prevents extension of non-specifically annealed primers and primer-dimers formed at low temperatures during qPCR setup.



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Products
Info
Data Sheet
Cat. No. Pack Size Conc.
08-25-00008 8 ml 5x
08-25-00020 20 ml 5x
08-25-00001 1 ml 5x
Applications:

- Detection and quantification of DNA and cDNA targets

- Profiling gene expression

- Microbial detection

- Viral load determination

Mix Compatibility:

- Bio-Rad: CFX96TM & CFX384TM, iQTM5 & MyiQTM, Chromo4TM, Opticon® 2 & MiniOpticon®

- Qiagen: Rotor-Gene® Q, Rotor-Gene® 6000

- Eppendorf: Mastercycler®: ep realplex2 & ep realplex4

- Illumina: The EcoTM

- Roche: LightCycler® 480

Mix Composition:

- HOT FIREPol® DNA Polymerase

- 5x EvaGreen® qPCR buffer

- 12.5 mM MgCl2 1x PCR solution – 2.5 mM

- dNTPs

- EvaGreen® dye

- No ROX dye

EvaGreen® Dye:

EvaGreen® is a DNA-binding dye with many features that make it a superior alternative to SYBR® Green I for qPCR. Apart from having similar spectra, EvaGreen® has three important features that set it apart from SYBR® Green I: EvaGreen® has much less PCR inhibition, is extremely stable dye and has been shown to be nonmutagenic and noncytotoxic. EvaGreen® is compatible with all common real-time PCR cyclers – simply select the standard settings for SYBR® Green or FAM!

Shipping and Storage Conditions:

Shipping and storage at room temperature has no detrimental effects on the quality 5x HOT FIREPol® EvaGreen® qPCR Mix Plus (no ROX). However you may store at -20°C for your own convinence.

For in vitro use only