5x HOT FIREPol® EvaGreen® qPCR Mix Plus (ROX)

For in vitro use only


5x HOT FIREPol® EvaGreen® qPCR Mix Plus (ROX) is an optimised ready-to-use solution for real-time quantitative PCR assays, including EvaGreen® dye. It comprises of all the components necessary to perform qPCR: HOT FIREPol® DNA Polymerase, ultrapure dNTPs, MgCl2, EvaGreen® dye and ROX dye. The user simply needs to add water, template and primers. HOT FIREPol® DNA Polymerase is activated by a 15 min incubation step at 95°C. This prevents extension of non-specifically annealed primers and primer-dimers formed at low temperatures during qPCR setup.

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Data Sheet
Cat. No. Pack Size Conc.
08-24-00008 8 ml 5x
08-24-00020 20 ml 5x
08-24-00001 1 ml 5x

- Detection and quantification of DNA and cDNA targets

- Profiling gene expression

- Microbial detection

- Viral load determination

Mix Compatibility:

- Applied BioSystems: QuantStudioTM 12K Flex, ViiATM 7, 7900HT, 7500, 7700, StepOneTM & StepOnePlusTM

- Stratagene: MX3000PTM , MX3005PTM

Mix Composition:

- HOT FIREPol® DNA Polymerase

- 5x EvaGreen® qPCR buffer

- 12.5 mM MgCl2 1x PCR solution – 2.5 mM

- dNTPs

- EvaGreen® dye

- ROX dye

EvaGreen® Dye:

EvaGreen® is a DNA-binding dye with many features that make it a superior alternative to SYBR® Green I for qPCR. Apart from having similar spectra, EvaGreen® has three important features that set it apart from SYBR® Green I: EvaGreen® has much less PCR inhibition, is extremely stable dye and has been shown to be nonmutagenic and noncytotoxic. EvaGreen® is compatible with all common real-time PCR cyclers – simply select the standard settings for SYBR® Green or FAM!

ROX Dye:

ROX is an internal passive reference dye used to normalize the fluorescent reporter signal generated in qPCR. It does not have any inhibitory effect on cyclers that do not need normalization.

Shipping and Storage Conditions:

Shipping and storage at room temperature has no detrimental effects on the quality 5x HOT FIREPol® EvaGreen® qPCR Mix Plus (ROX). However you may store at -20°C for your own convinence.

For in vitro use only