FIREPol® DNA Polymerase


For in vitro use only


Description:

FIREPol® is a highly processive, thermostable DNA polymerase. Due to its genetic modifications FIREPol® has an enhanced stability at room temperature with no activity loss for up to 1 month. The enzyme has 5′ → 3′ polymerization-dependent exonuclease replacement activity but lacks 3′ → 5′ exonuclease activity.



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Products
Info
Data Sheet
Cat. No. Pack Size Conc.
01-01-01000 1000 U 5 U/µl
01-01-02000 2000 U 5 U/µl
01-01-00500 500 U 5 U/µl
Applications:

- Suited for a wide range of PCR assays

- Primer extension

- TA cloning

Concentration:

5 U/µl

Storage and Dilution Buffer:

50% glycerol (v/v), 20 mM Tris-HCl pH 8.7 at 25°C, 100 mM KCl, 0.1 mM EDTA and stabilizers.

Reagents Provided:

- FIREPol® DNA Polymerase

- 10x Buffer B(Mg2+ free)
0.8 M Tris-HCl, 0.2 M (NH4)2SO4, 0.2% w/v Tween-20

- 10x Buffer BD(Mg2+, detergent free)
0.8 M Tris-HCl, 0.2 M (NH4)2SO4

- 25 mM MgCl2

- 10x Solution S Additive that facilitates amplification of difficult templates (e.g. GC-rich DNA templates). This solution should be used at a defined working concentration (1x, 2x or 3x solution).
Solution S is NOT a reaction buffer and should be used ONLY IF non-specific amplifications occur.

Shipping and Storage Conditions:

Shipping and storage at room temperature has no detrimental effects on the quality FIREPol® DNA Polymerase. However you may store at -20°C for your own convinence.

For in vitro use only