For in vitro use only
Description:
FIREPol® is a highly processive, thermostable DNA polymerase. Due to its genetic modifications FIREPol® has an enhanced stability at room temperature with no activity loss for up to 1 month. The enzyme has 5′ → 3′ polymerization-dependent exonuclease replacement activity but lacks 3′ → 5′ exonuclease activity.
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Cat. No. | Pack Size | Conc. |
---|---|---|
01-01-01000 | 1000 U | 5 U/µl |
01-01-02000 | 2000 U | 5 U/µl |
01-01-00500 | 500 U | 5 U/µl |
- Suited for a wide range of PCR assays
- Primer extension
- TA cloning
5 U/µl
50% glycerol (v/v), 20 mM Tris-HCl pH 8.7 at 25°C, 100 mM KCl, 0.1 mM EDTA and stabilizers.
- FIREPol® DNA Polymerase
- 10x Buffer B(Mg2+ free)
0.8 M Tris-HCl, 0.2 M (NH4)2SO4, 0.2% w/v Tween-20
- 10x Buffer BD(Mg2+, detergent free)
0.8 M Tris-HCl, 0.2 M (NH4)2SO4
- 25 mM MgCl2
- 10x Solution S Additive that facilitates amplification of difficult templates (e.g. GC-rich DNA templates). This solution should be used at a defined working concentration (1x, 2x or 3x solution).
Solution S is NOT a reaction buffer and should be used ONLY IF non-specific amplifications occur.
Shipping and storage at room temperature has no detrimental effects on the quality FIREPol® DNA Polymerase. However you may store at -20°C for your own convinence.
For in vitro use only
Some applications this product is used in may require a license which is not provided by the purchase of this product. Users should obtain the license if required.
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