5x FIREPol® Master Mix Ready to Load


For in vitro use only


Description:

5x FIREPol® Master Mix is a premixed ready-to-use solution containing all reagents required for PCR (except template, primers and water). Ready to Load format also includes a compound needed for direct loading onto agarose gel and two tracking dyes (blue and yellow) that allow to monitor progress during electrophoresis. We recommend using 5x FIREPol® Master Mix Ready to Load in any PCR application that will be visualized by agarose gel electrophoresis and ethidium bromide staining. 5x FIREPol® Master Mix Ready to Load is not recommended for use in post-PCR applications where spectro-photometric measurements (absorbance or fluorescence) are necessary because yellow and blue dyes can interfere with these applications.



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This Product is offered with an option of 2 different MgCl2 Concentrations:

  • -7.5 MgCl2- 1x PCR solution - 1.5 mM
  • -12.5 MgCl2- 1x PCR solution - 2.5 mM
Products
Info
Data Sheet
Cat. No. Pack Size Conc.
04-12-00125 1 ml 12.5 mM MgCl2
04-12-00115 1 ml 7.5 mM MgCl2
Applications:

- Suited for a wide range of PCR assays

- Primer extension

Reagent Composition:

- FIREPol® DNA Polymerase

- 5x Buffer B(Mg2+ free)
0.4 M Tris-HCl, 0.1 M (NH4)2SO4, 0.1% w/v Tween-20

- 7.5 mM MgCl2 1x PCR solution – 1.5 mM

- 12.5 mM MgCl2 1x PCR solution – 2.5 mM

- 2 mM dNTPs of each 1x PCR solution – 200 µM dATP, 200 µM dCTP, 200 µM dGTP and 200 µM dTTP

- Blue dye Migration equivalent to 3.5-4.5 kb DNA fragment

- Yellow dye Migration rate in excess of primers in 1% agarose gel: <35-45 bp

- Compound that increases sample density for direct loading

Advantages of Ready-to-Load Mix

- decreased set-up time

- reduced risk of contamination

- consistent reaction to reaction performance

- less pipetting mistakes

- contains all reagents required for PCR

Like all our products, the 5x FIREPol® Master Mix Ready to Load is stable at ambient temperature (up to 37°C). This makes handling and set-up even more convenient, since no ice has to be used.

Shipping and Storage Conditions:

Shipping and storage at room temperature has no detrimental effects on the quality 5x FIREPol® Master Mix Ready to Load. However you may store at -20°C for your own convinence.

For in vitro use only