HOT TERMIPol® DNA Polymerase


For in vitro use only


Description:

HOT TERMIPol® DNA Polymerase is a thermostable DNA polymerase suited for MALDI-TOF mass spectrometry and other primer extension platforms. The enzyme has 5′ → 3′ polymerase activity and enhanced efficiency for incorporating unconventional nucleotides (ddNTPs and labeled ddNTPs). HOT TERMIPol® DNA polymerase is a chemically modified TERMIPol® DNA polymerase. At ambient temperatures it is inactive, having no polymerase activity. HOT TERMIPol® DNA polymerase is fully activated by a 8-12 minute incubation at 95°C. This prevents extension of non-specifically annealed primers.



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Products
Info
Data Sheet
Cat. No. Pack Size Conc.
01-06-00500 500 U 5 U/µl
Applications:

- Primer extension

- MALDI-TOF mass spectrometry

- Mass-array

Concentration:

5 U/µl

Reagents Provided:

- HOT TERMIPol® DNA Polymerase

- 10x Reaction Buffer C
500 mM Tris-HCl pH 9.5 at 25°C

- 100 mM MgCl2

Storage and Dilution Buffer:

50% glycerol (v/v), 20 mM Tris-HCl pH 8.7 at 25°C, 100 mM KCl, 0.1 mM EDTA and stabilizers.

Shipping and Storage Conditions:

Shipping and storage at room temperature has no detrimental effects on the quality HOT TERMIPol® DNA Polymerase. However you may store at -20°C for your own convinence.

For in vitro use only