5x HOT FIREPol® EvaGreen® HRM Mix (ROX)

For in vitro use only


5x HOT FIREPol® EvaGreen® HRM Mix (ROX) is an optimised ready-to-use solution for High Resolution Melt (HRM) Analysis, incorporating EvaGreen® dye. It comprises all the components necessary to perform qPCR and HRM Analysis: HOT FIREPol® DNA Polymerase, ultrapure dNTPs, MgCl2, EvaGreen® dye and ROX dye according to system requirements. The user simply needs to add water, template and primers. HOT FIREPol® DNA Polymerase is activated by a 15 min incubation step at 95°C. This prevents extension of non-specifically annealed primers and primer-dimers formed at low temperatures during qPCR setup.

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Data Sheet
Cat. No. Pack Size Conc.
08-33-00001 1 ml 5x
08-33-00008 8 ml 5x
08-33-00020 20 ml 5x

- High Resolution Melt (HRM)

Mix Compatibility:

- Applied BioSystems: QuantStudioTM 12K Flex, ViiATM 7, 7900HT, 7500(with new HRM software v. 2.0)

Mix Composition:

- HOT FIREPol® DNA Polymerase

- 5x EvaGreen® HRM buffer

- 12.5 mM MgCl2 1x PCR solution – 2.5 mM

- dNTPs

- EvaGreen® dye


- ROX dye

EvaGreen® Dye:

EvaGreen® is a DNA-binding dye with many features that make it a superior alternative to SYBR® Green I for qPCR. Apart from having similar spectra, EvaGreen® has three important features that set it apart from SYBR® Green I: EvaGreen® has much less PCR inhibition, is extremely stable dye and has been shown to be nonmutagenic and noncytotoxic. EvaGreen® is compatible with all common real-time PCR cyclers – simply select the standard settings for SYBR® Green or FAM!

Shipping and Storage Conditions:

Shipping and storage at room temperature has no detrimental effects on the quality 5x HOT FIREPol® EvaGreen® HRM Mix (ROX). However you may store at -20°C for your own convinence.

For in vitro use only