HOT FIREPol® DNA Polymerase

HOT START DNA Polymerase

For in vitro use only


HOT FIREPol® DNA Polymerase is a chemically modified FIREPol® DNA Polymerase. HOT FIREPol® DNA Polymerase is activated by a 15 min incubation step at 95°C. This prevents extentension of non-specifically annealed primers and primer-dimers formed at low temperatures during PCR setup. The enzyme has 5′ → 3′ polymerization-dependent exonuclease replacement activity but lacks 3′ → 5′ exonuclease activity.

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Data Sheet
Cat. No. Pack Size Conc.
01-02-00500 500 U 5 U/µl
01-02-01000 1000 U 5 U/µl

- Hot Start PCR

- Primer extension

- TA cloning


5 U/µl

Reagents Provided:

- HOT FIREPol® DNA Polymerase

- HOT FIREPol® 10x Buffer B1(Mg2+, detergent free)
Tris-HCl and (NH4)2SO4

- HOT FIREPol® 10x Buffer B2(Mg2+ free)
Tris-HCl, (NH4)2SO4 and detergent

- 25 mM MgCl2

- 10x Solution S Additive that facilitates amplification of difficult templates (e.g. GC-rich DNA templates). This solution should be used at a defined working concentration (1x, 2x or 3x solution).
Solution S is NOT a reaction buffer and should be used ONLY IF non-specific amplifications occur.

Storage and Dilution Buffer:

50% glycerol (v/v), 20 mM Tris-HCl pH 8.7 at 25°C, 100 mM KCl, 0.1 mM EDTA and stabilizers.

Shipping and Storage Conditions:

Shipping and storage at room temperature has no detrimental effects on the quality HOT FIREPol® DNA Polymerase. However you may store at -20°C for your own convinence.

For in vitro use only