5x HOT FIREPol® Blend Master Mix Ready to Load


For in vitro use only


Description:

5x HOT FIREPol® Blend Master Mix Ready to Load is a premixed ready-to-use solution containing all reagents required for PCR (except template, primers and water), additional compound needed for direct loading onto agarose gel and two tracking dyes (blue and yellow) that allow to monitor progress during electrophoresis. HOT FIREPol® Blend Master Mix contains two carefully optimized enzymes – HOT FIREPol® DNA polymerase and a proofreading polymerase. This enzyme blend has both the 5′ → 3′ exonuclease activity as well as the 3′ → 5′ proofreading activity. HOT FIREPol® Blend Master Mix exhibits an increased fidelity (up to five fold) compared to regular Taq polymerase. In addition, the mix also allows amplification of longer fragments. Generated PCR products are compatible with blunt-end cloning procedures.



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This Product is offered with an option of 4 different MgCl2 Concentrations:

  • -7.5 MgCl2- 1x PCR solution - 1.5 mM
  • -10 MgCl2- 1x PCR solution - 2 mM
  • -12.5 MgCl2- 1x PCR solution - 2.5 mM
  • -15 MgCl2- 1x PCR solution - 3 mM
Products
Info
Data Sheet
Cat. No. Pack Size Conc.
04-25-02020 20 ml 10 mM MgCl2
04-25-00120 1 ml 10 mM MgCl2
04-25-02025 20 ml 12.5 mM MgCl2
04-25-00125 1 ml 12.5 mM MgCl2
04-25-02030 20 ml 15 mM MgCl2
04-25-00130 1 ml 15 mM MgCl2
04-25-02015 20 ml 7.5 mM MgCl2
04-25-00115 1 ml 7.5 mM MgCl2
Applications:

- Hot Start PCR

Mix Composition:

- HOT FIREPol® DNA Polymerase

- Proofreading enzyme

- 5x Blend Master Mix Buffer

- 7.5 mM MgCl2 1x PCR solution – 1.5 mM

- 10 mM MgCl2 1x PCR solution – 2 mM

- 12.5 mM MgCl2 1x PCR solution – 2.5 mM

- 15 mM MgCl2 1x PCR solution – 3 mM

- 2 mM dNTPs of each 1x PCR solution – 200 µM dATP, 200 µM dCTP, 200 µM dGTP and 200 µM dTTP

- BSA

- Blue dye Migration equivalent to 3.5-4.5 kb DNA fragment

- Yellow dye Migration rate in excess of primers in 1% agarose gel: <35-45 bp

- Compound that increases sample density for direct loading

Advantages of Ready-to-Load Mix:

- decreased set-up time

- reduced risk of contamination

- consistent reaction to reaction performance

- less pipetting mistakes

- contains all reagents required for PCR

Like all our products, the 5x HOT FIREPol® Blend Master Mix Ready to Load is stable at ambient temperature (up to 37°C). This makes handling and set-up even more convenient, since no ice has to be used.

Shipping and Storage Conditions:

Shipping and storage at room temperature has no detrimental effects on the quality 5x HOT FIREPol® Blend Master Mix Ready to Load. However you may store at -20°C for your own convinence.

For in vitro use only